LOCUS       sequence-108321-         533 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC77
FEATURES             Location/Qualifiers
     source          1..533
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..23
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             288..473
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
ORIGIN
        1 tgcccaaaaa gaaaagaaaa gtgggtagta tgaaatcaat tcgctgtaaa aactgcaaca
       61 aactgttatt taaggcggat agttttgatc acattgaaat caggtgtccg cgttgcaaac
      121 gtcacatcat aatgctgaat gcctgcgagc atcccacgga gaaacattgt gggaaaagag
      181 aaaaaatcac gcattctgac gaaaccgtgc gttatggctc cactagtggg cacaagctta
      241 atggcggtgg cggagggatg gatgctaagt cactaactgc ctggtcccgg acactggtga
      301 ccttcaagga tgtatttgtg gacttcacca gggaggagtg gaagctgctg gacactgctc
      361 agcagatcgt gtacagaaat gtgatgctgg agaactataa gaacctggtt tccttgggtt
      421 atcagcttac taagccagat gtgatcctcc ggttggagaa gggagaagag ccctagcccg
      481 ggtcgagcat gcatctaggg cggccaattc cgcccctctc cctccccccc ccc
//
