LOCUS       sequence-108325-         647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA for mammalian cells with mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC103
FEATURES             Location/Qualifiers
     source          1..647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        257..560
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtatcccttg gagaaccacc ttgttggaat agctcagagg ccgagggttt aagagctatg
       61 ctggaaacag catagcaagt ttaaataagg ctagtccgtt atcaacttga aaaagtggca
      121 ccgagtcggt gctttttttc tcgagtacta ggatccatta ggcggccgcg tggataaccg
      181 tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      241 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      301 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      361 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      421 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      481 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      541 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      601 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaat
//
