LOCUS       sequence-108331-         513 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA + 1x COM with COM-KRAB effector for mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC74
FEATURES             Location/Qualifiers
     source          1..513
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             268..453
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
ORIGIN
        1 gtgggtagta tgaaatcaat tcgctgtaaa aactgcaaca aactgttatt taaggcggat
       61 agttttgatc acattgaaat caggtgtccg cgttgcaaac gtcacatcat aatgctgaat
      121 gcctgcgagc atcccacgga gaaacattgt gggaaaagag aaaaaatcac gcattctgac
      181 gaaaccgtgc gttatggctc cactagtggg cacaagctta atggcggtgg cggagggatg
      241 gatgctaagt cactaactgc ctggtcccgg acactggtga ccttcaagga tgtatttgtg
      301 gacttcacca gggaggagtg gaagctgctg gacactgctc agcagatcgt gtacagaaat
      361 gtgatgctgg agaactataa gaacctggtt tccttgggtt atcagcttac taagccagat
      421 gtgatcctcc ggttggagaa gggagaagag ccctagcccg ggtcgagcat gcatctaggg
      481 cggccaattc cgcccctctc cctccccccc ccc
//
