LOCUS       sequence-108333-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the mouse Mars gene with the L274G mutation 
            under a CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Mahdavi A, Hamblin GD, Jindal GA, Bagert JD, Dong C, Sweredoski MJ, 
            Hess S, Schuman EM, Tirrell DA
  TITLE     Engineered Aminoacyl-tRNA Synthetase for Cell-Selective Analysis of 
            Mammalian Protein Synthesis.
  JOURNAL   J Am Chem Soc. 2016 Apr 6;138(13):4278-81. doi: 
            10.1021/jacs.5b08980. Epub 2016 Mar 25.
  PUBMED    26991063
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.5b08980"; journalName: "J Am Chem Soc"; date: 
            "2016-04-6- 6"; volume: "138"; issue: "13"; pages: "4278-81"
COMMENT     Sequence Label: pMarsL274G
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             84..101
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
       61 agctggctag cgccaccatg gcccaccatc accatcacca tatgagactg ttcgtgagcg
      121 agggttcccc ggggagcctg cccgtgctgg ctgcggccgc gagggcccgg ggtcgggcgg
      181 agctgctcat cagcaccgta ggccccgaag agtgtgtggt accattcctt acccggccta
      241 aggtccctgt cttgcagctg gatagtggca actacctctt ctctgctagt gcaatctgcc
      301 gatatttttt tctgttatgt ggctgggaac aagatgatct caccaaccag tggctggaat
      361 gggaggcaac agaactgcag ccagttctgt ctgctgccct acactgtcta gtggttcaag
      421 gcaagaaagg ggaagatata cttggcccac ttcggagagt cctgactcac attgatcaca
      481 gcttgagtcg tcaaaactgt cctttcctgg ctggggacac agaatctcta gctgacattg
      541 ttttgtgggg agcactgtat cctttactgc aagacccagc ttacctccct gaggagttgg
      601 gtgccctgca aagttggttc cagacactga gtacccagga accgtgtcag cgagctgcag
      661 agacggtgct aaaacagcag ggtgtcctgg cacttcgtct gtacctccag aaacagccac
      721 agcctcagcc cccgcctcct gaggggagaa ctgtcagcaa cgagctggag gaagaggaac
      781 tggctacctt gtctgaggag gacatcgtta cagctgttgc cgcgtgggag aagggtctgg
      841 aaagctgctc cctaactca
//
