LOCUS       sequence-108335-         591 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA + 1x MS2 with MCP-VP64 effector for mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC25
FEATURES             Location/Qualifiers
     source          1..591
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             376..525
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 gtgggtagta tggcttctaa ctttactcag ttcgttctcg tcgacaatgg cggaactggc
       61 gacgtgactg tcgccccaag caacttcgct aacgggatcg ctgaatggat cagctctaac
      121 tcgcgttcac aggcttacaa agtaacctgt agcgttcgtc agagctctgc gcagaatcgc
      181 aaatacacca tcaaagtcga ggtgcctaaa ggcgcctggc gttcgtactt aaatatggaa
      241 ctaaccattc caattttcgc cacgaattcc gactgcgagc ttattgttaa ggcaatgcaa
      301 ggtctcctaa aagatggaaa cccgattccc tcagcaatcg cagcaaactc cggcatctac
      361 ggctccggac gagccgatgc tctcgacgat tttgacctcg atatgctggg atccgacgct
      421 ctcgatgact tcgaccttga catgctgggg agtgacgctc tggacgactt cgatctcgac
      481 atgctgggct ccgacgccct ggatgacttc gatctggaca tgctcatcaa ctgacccggg
      541 tcgagcatgc atctagggcg gccaattccg cccctctccc tccccccccc c
//
