LOCUS       sequence-108336-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 505, Emission: 515. This 
            plasmid encodes dClover2 A206K, H231L based on publication..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: dClover2-Cx43-7
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(137..856)
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNSIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(182..203)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     790..811
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      853..862
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agctgcataa cagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
       61 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
      121 ctagagtcgc ggccgcttac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
      181 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg
      241 actgatggct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
      301 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc aacgttgtgg cggatcttga
      361 agttagcctt gatgctgttc ttctgcttgt cggccgtgat atagacgtag tggctgttga
      421 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
      481 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagg
      541 taccgtcgtc cttgaaagag atggtgcgct cctggacgta gccttcgggc atggcggact
      601 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcaggcc acgccgtagc
      661 cgaaggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
      721 tcagggtcag cttgccgttg gtggcatcgc cctcgccctc gccgcggacg ctgaacttgt
      781 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct
      841 cgcccttgct caccatggtg gcgaccggtg gatccgc
//
