LOCUS       sequence-108337-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the mouse Mars gene with the L274G mutation 
            under a CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Mahdavi A, Hamblin GD, Jindal GA, Bagert JD, Dong C, Sweredoski MJ, 
            Hess S, Schuman EM, Tirrell DA
  TITLE     Engineered Aminoacyl-tRNA Synthetase for Cell-Selective Analysis of 
            Mammalian Protein Synthesis.
  JOURNAL   J Am Chem Soc. 2016 Apr 6;138(13):4278-81. doi: 
            10.1021/jacs.5b08980. Epub 2016 Mar 25.
  PUBMED    26991063
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.5b08980"; journalName: "J Am Chem Soc"; date: 
            "2016-04-6- 6"; volume: "138"; issue: "13"; pages: "4278-81"
COMMENT     Sequence Label: pMarsL274G
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             390..401
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(642..653)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 tcgagttatc actttttctt cttgccttta ggagtttcaa tgggtttccc ctcagccaaa
       61 gccaactgtt tctttagatc caagagttta gccacctctg cagcaacctg gttcttgtct
      121 gccttctgtg ctttcagttc ccggacgacg ttgccctgct tggtcacctc atccgtcagc
      181 gtttgtatgt gctgcgagcc tgctgctgta actgcctcca cagctgctgg cttgggggag
      241 cctttagcct gaccccctcc aaagcgctgc ctcaaatttt caatctggtc attttccagt
      301 ttttggaaca aaggactgac tgtgccaatt cggtggcctg ctggcaaggt acaaatgaag
      361 cttgtggcaa ggatgcggca ggctgcaggt gggagctgca gctgggtctg gatggtagag
      421 ctgactgtgg gcatgtatgg ctgcagcatg acagacagca aggcagccat gttcactgcc
      481 atgcctgtca ctgtgcctgc ccgctgcctg tccatctcac cacctttaat ccgtttccag
      541 ggctcattca cttgaatgta ttggttgcca tggcgagata tggtgaggat actgcgcaag
      601 gcatcccgga tccgaacctt ttccaacagc tggtgatagt gctggagttc ccaagagaca
      661 tgggccacca ggcgtctgtc atcaggggtt agcgccatct caggcacaca accgccaaaa
      721 aacttagaaa caaacatgcc agctctgttg atgaagttgc ccaggttgtt gagcagctca
      781 gaattgtttt taatcaacaa gtctgtccag gag
//
