LOCUS       sequence-108338-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast dCas9-VP64 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC519
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(136..156)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(163..312)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(319..339)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(355..375)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcattttat agagtatata caactgtata agatagtaat aaaaatatcg cactcacgta
       61 aacacttaat cataatatta attcgtatat aatttagcta tttgcttaga gctccaccgc
      121 ggtggcggcc gcctacactt tcctcttctt tttgggacta cctagcatat ctagatcaaa
      181 gtcatctagg gcatcggagc ccaacatatc taaatcgaag tcgtccaatg catctgagcc
      241 taacatgtct aggtcgaagt cgtctaacgc gtctgaacca agcatatcta agtcaaaatc
      301 gtctaaagca tcactaccaa ctttgcgttt ctttttcgga gatccggaac tacctacctt
      361 gcgctttttc ttgggagctc cctcatcccc tccgagctgt gagaggtcaa tccgagtttc
      421 gtaaagtccg gtgatgctct ggtggatgag ggtggcgtcc agcacttctt tggtggaggt
      481 atagcgtttc cggtcgatgg tggtgtcgaa gtacttgaat gcggcagggg caccaagatt
      541 ggtgagggtg aacaggtgga tgatattctc agcctgctcc cgaattggtt tgtctctgtg
      601 cttattgtat gcgctaagga ctttatcgag gttggcatca gcgagaatga cgcgtttaga
      661 aaactcggaa atctgctcaa tgatctcgtc cagataatgc ttatgctgct ccacaaacag
      721 ttgcttttgc tcgttgtcct c
//
