LOCUS       sequence-108339-         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MCP-VP64 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC522
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(142..291)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(664..684)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcattttat agagtatata caactgtata agatagtaat aaaaatatcg cactcacgta
       61 aacacttaat cataatatta attcgtatat aatttagcta tttgcttaga gctccaccgc
      121 ggtggcggcc gctcagttga tgagcatgtc cagatcgaag tcatccaggg cgtcggagcc
      181 cagcatgtcg agatcgaagt cgtccagagc gtcactcccc agcatgtcaa ggtcgaagtc
      241 atcgagagcg tcggatccca gcatatcgag gtcaaaatcg tcgagagcat cggctcgtcc
      301 ggagccgtag atgccggagt ttgctgcgat tgctgaggga atcgggtttc catcttttag
      361 gagaccttgc attgccttaa caataagctc gcagtcggaa ttcgtggcga aaattggaat
      421 ggttagttcc atatttaagt acgaacgcca ggcgccttta ggcacctcga ctttgatggt
      481 gtatttgcga ttctgcgcag agctctgacg aacgctacag gttactttgt aagcctgtga
      541 acgcgagtta gagctgatcc attcagcgat cccgttagcg aagttgcttg gggcgacagt
      601 cacgtcgcca gttccgccat tgtcgacgag aacgaactga gtaaagttag aagccatact
      661 acccactttt cttttctttt tgggcatctc gagagacatt gtatatgaga tagttgattg
      721 tatgcttggt atagcttgaa atattgtgca g
//
