LOCUS       sequence-108342-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC620
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(146..166)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(170..190)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(194..214)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcattttat agagtatata caactgtata agatagtaat aaaaatatcg cactcacgta
       61 aacacttaat cataatatta attcgtatat aatttagcta tttgcttaga gctccaccgc
      121 ggtggcggcc gccggtagaa ttctatacct ttctcttctt ttttggatct acctttctct
      181 tcttttttgg atctaccttt ctcttctttt ttggatcagc tccctcatcc cctccgagct
      241 gtgagaggtc aatccgagtt tcgtaaagtc cggtgatgct ctggtggatg agggtggcgt
      301 ccagcacttc tttggtggag gtatagcgtt tccggtcgat ggtggtgtcg aagtacttga
      361 atgcggcagg ggcaccaaga ttggtgaggg tgaacaggtg gatgatattc tcagcctgct
      421 cccgaattgg tttgtctctg tgcttattgt atgcgctaag gactttatcg aggttggcat
      481 cagcgagaat gacgcgttta gaaaactcgg aaatctgctc aatgatctcg tccagataat
      541 gcttatgctg ctccacaaac agttgctttt gctcgttgtc ctcggggctc cccttcagct
      601 tctcgtaatg gcttgcaagg tagaggaaat tcacgtactt ggaggggaga gccagctcat
      661 tacccttctg aagttcgcca gcggaggcga gcatccgctt ccgcccattc tccagttcga
      721 acaggctata ctttgggagc ttgatgataa ggtccttctt gac
//
