LOCUS       sequence-108343-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC620
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             280..429
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 atcgctgaat ggatcagctc taactcgcgt tcacaggctt acaaagtaac ctgtagcgtt
       61 cgtcagagct ctgcgcagaa tcgcaaatac accatcaaag tcgaggtgcc taaaggcgcc
      121 tggcgttcgt acttaaatat ggaactaacc attccaattt tcgccacgaa ttccgactgc
      181 gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat tccctcagca
      241 atcgcagcaa actccggcat ctacggctcc ggacgagccg atgctctcga cgattttgac
      301 ctcgatatgc tgggatccga cgctctcgat gacttcgacc ttgacatgct ggggagtgac
      361 gctctggacg acttcgatct cgacatgctg ggctccgacg ccctggatga cttcgatctg
      421 gacatgctca tcaactgaag tgcttttaac taagaattat tagtcttttc tgcttatttt
      481 ttcatcatag tttagaacac tttatattaa cgaatagttt atgaatctat ttaggtttaa
      541 aaattgatac agttttataa gttacttttt caaagactcg tgctgtctat tgcataatgc
      601 actggaaggg gaaaaaaaag gtgcacacgc gtggcttttt cttgaatttg cagtttgaaa
      661 aataactaca tggatgataa gaaaacatgg agtacagtca ctttgagaac cttcaatcag
      721 ctggtaacgt cttcgttaat tggatactc
//
