LOCUS       sequence-108348-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme 
            design for yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC625
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(578..645)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     misc_RNA        complement(652..670)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(675..750)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           complement(771..807)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
ORIGIN
        1 gtcaagactg tcaaggaggg tattctgggc ctccatgtcg ctggccgggt gacccggcgg
       61 ggacgaggca agctaaacag atctggacca atcaagcaat tcagaaactt tgacaaatct
      121 aataaactct aaagaaattg aaaacttgaa acttgaaaac accgagttga taactaactt
      181 aaataattta attatcaata atgaaaaact aacacaaaca atcaatgcca gagatcaaac
      241 cataaaatca ttaaccaaaa tcaacgacaa attgaataat aaaattgatg cattaaattt
      301 ggaaataaac gagaaaaaca aaactataga attgattaat gatgaagtat tgacaaatca
      361 aatacaattg aatgtattga gaagtaaact agagttgttg gagaggtaaa cccagaaagc
      421 attcatcgcg ttaattaata gacaactcat tttatagagt atatacaact gtataagata
      481 gtaataaaaa tatcgcactc acgtaaacac ttaatcataa tattaattcg tatataattt
      541 agctatttgc ttagagctcc accgcggtgg cggccgcgtc ccattcgcca tgccgaagca
      601 tgttgcccag ccggcgccag cgaggaggct gggaccatgc cggccaaaag cacatgggtg
      661 atcctcatgt gcgcgcaccg actcggtgcc actttttcaa gttgataacg gactagcctt
      721 attttaactt gctatttcta gctctaaaac tatcagtgat agagaaaagt gacgagctta
      781 ctcgtttcgt cctcacggac tcatcagac
//
