LOCUS       sequence-108352-         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MCP-VP64 and dCas9 in Yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC638
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             145..165
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             538..687
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 gtataaatag acctgcaatt attaatcttt tgtttcctcg tcattgttct cgttcccttt
       61 cttccttgtt tctttttctg cacaatattt caagctatac caagcataca atcaactatc
      121 tcatatacaa tgtctctcga gatgcccaaa aagaaaagaa aagtgggtag tatggcttct
      181 aactttactc agttcgttct cgtcgacaat ggcggaactg gcgacgtgac tgtcgcccca
      241 agcaacttcg ctaacgggat cgctgaatgg atcagctcta actcgcgttc acaggcttac
      301 aaagtaacct gtagcgttcg tcagagctct gcgcagaatc gcaaatacac catcaaagtc
      361 gaggtgccta aaggcgcctg gcgttcgtac ttaaatatgg aactaaccat tccaattttc
      421 gccacgaatt ccgactgcga gcttattgtt aaggcaatgc aaggtctcct aaaagatgga
      481 aacccgattc cctcagcaat cgcagcaaac tccggcatct acggctccgg acgagccgat
      541 gctctcgacg attttgacct cgatatgctg ggatccgacg ctctcgatga cttcgacctt
      601 gacatgctgg ggagtgacgc tctggacgac ttcgatctcg acatgctggg ctccgacgcc
      661 ctggatgact tcgatctgga catgctcatc aactgaagtg cttttaacta agaattatta
      721 gtcttttctg ctta
//
