LOCUS       sequence-108355-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide 
            RNA which target the NDM-1 gene. Contains an R1162 origin and 
            chloramphenicol resistance. Can be mobilized by S17-1 lambda pir..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Citorik RJ, Mimee M, Lu TK
  TITLE     Sequence-specific antimicrobials using efficiently delivered 
            RNA-guided nucleases.
  JOURNAL   Nat Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011.
  PUBMED    25240928
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011."
COMMENT     Sequence Label: pMM441
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(42..120)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     promoter        join(285..303,304..309,310..326,327..332,333..358)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    285..303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    310..328
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             368..379
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             394..411
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttttggtttg cagtcagagt agaatagaag tatcaaaaaa agcaccgact cggtgccact
       61 ttttcaagtt gataacggac tagccttatt ttaacttgct atgctgtttt gaatggttcc
      121 aacaagatta ttttataact tttataacaa ataatcaagg agaaattcaa agaaatttat
      181 cagccataaa acaatactta atactataga atgataacaa aataaactac tttttaaaag
      241 aattttgtgt tataatctat ttattattaa gtattgggat cgattcccta tcagtgatag
      301 agattgacat ccctatcagt gatagagata ctgagcacat cagcaggacg cactgaccga
      361 attcattaaa gaggagaaag gtgcggccgc atgcatcacc atcaccatca catggataag
      421 aaatactcaa taggcttaga tatcggcaca aatagcgtcg gatgggcggt gatcactgat
      481 gaatataagg ttccgtctaa aaagttcaag gttctgggaa atacagaccg ccacagtatc
      541 aaaaaaaatc ttataggggc tcttttattt gacagtggag agacagcgga agcgactcgt
      601 ctcaaacgga cagctcgtag aaggtataca cgtcggaaga atcgtatttg ttatctacag
      661 gagatttttt caaatgagat ggcgaaagta gatgatagtt tctttcatcg acttgaagag
      721 tcttttttgg tggaagaaga caagaagcat gaacgtcatc ctatttttgg aaatatagta
      781 gatgaagttg cttatcatga gaaatatcca actatctatc atctgcgaaa a
//
