LOCUS       sequence-108356-         667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide 
            RNA which target the NDM-1 gene. Contains an R1162 origin and 
            chloramphenicol resistance. Can be mobilized by S17-1 lambda pir..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Citorik RJ, Mimee M, Lu TK
  TITLE     Sequence-specific antimicrobials using efficiently delivered 
            RNA-guided nucleases.
  JOURNAL   Nat Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011.
  PUBMED    25240928
REFERENCE   2  (bases 1 to 667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011."
COMMENT     Sequence Label: pMM441
FEATURES             Location/Qualifiers
     source          1..667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    37..168
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   169..204
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   235..270
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
ORIGIN
        1 ctatatgatt ttccgcagta tattttagat gaagattatt tcttaataac taaaaatatg
       61 gtataatact cttaataaat gcagtaatac aggggctttt caagactgaa gtctagctga
      121 gacaaatagt gcgattacga aattttttag acaaaaatag tctacgaggt tttagagcta
      181 tgctgttttg aatggtccca aaacgggcag tcgcttccaa cggtttgatc gtcagtttta
      241 gagctatgct gttttgaatg gtcccaaaac ttcagcacac tgagacttgt tgagttccat
      301 gttttagagc tatgctgttt tgaatggtct ccattctcta gatgatgatt aaggatctat
      361 ttttttgggc ggggccgccc aaaaaaatgg ttcgcttgct gtcccccggg gacagacgtc
      421 atactagata tcaagcgact tctcctatcc cctgggaaca catcaatctc accggagaat
      481 atcgctggcc aaagccttag cgtaggattc cgccccttcc cgcaaacgac cccaaacagg
      541 aaacgcagct gaaacgggaa gctcaacacc cactgacgca tgggttgttc aggcagtact
      601 tcatcaacca gcaaggcggc actttcggcc atccgccgcg ccccacagct cgggcagaaa
      661 ccgcgac
//
