LOCUS       sequence-108358-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide 
            RNA which target the NDM-1 gene. Contains a pBBR1 origin and 
            chloramphenicol resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Citorik RJ, Mimee M, Lu TK
  TITLE     Sequence-specific antimicrobials using efficiently delivered 
            RNA-guided nucleases.
  JOURNAL   Nat Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011.
  PUBMED    25240928
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011."
COMMENT     Sequence Label: pMM178
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   203..238
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   269..304
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     misc_feature    complement(305..436)
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     terminator      complement(503..589)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc
       61 ggttttatgg acagcaagcg aaccattttt ttgggcggcc ccgcccaaaa aaatagatcc
      121 ttaatcatca tctagagaat ggagaccatt caaaacagca tagctctaaa acatggaact
      181 caacaagtct cagtgtgctg aagttttggg accattcaaa acagcatagc tctaaaactg
      241 acgatcaaac cgttggaagc gactgcccgt tttgggacca ttcaaaacag catagctcta
      301 aaacctcgta gactattttt gtctaaaaaa tttcgtaatc gcactatttg tctcagctag
      361 acttcagtct tgaaaagccc ctgtattact gcatttatta agagtattat accatatttt
      421 tagttattaa gaaataatct tcatctaaaa tatactgcgg aaaatcatat agttctgcag
      481 ggtgctaggt ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
      541 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctctagc
      601 tcgagtcagt cacctcctag ctgactcaaa tcaatgcgtg tttcataaag accagtgatg
      661 gattgatgga taagagtggc atctaaaact tcttttgtag acgtatatcg tttacgatca
      721 attgttgtat caaaatattt aaaagcagcg ggagctccaa gattcgtcaa cgtaaataaa
      781 tgaataatat tttctgcttg ttcacgtatt ggtttgtctc tatgtttgtt atatgcacta
      841 agaactttat ctaaattggc atctgctaaa ataacacgct tagaaaattc actgatttgc
      901 tcaataatc
//
