LOCUS       sequence-108360-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide 
            RNA which target the NDM-1 gene. Contains a pBBR1 origin and 
            chloramphenicol resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Citorik RJ, Mimee M, Lu TK
  TITLE     Sequence-specific antimicrobials using efficiently delivered 
            RNA-guided nucleases.
  JOURNAL   Nat Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011.
  PUBMED    25240928
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011."
COMMENT     Sequence Label: pMM178
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(44..122)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     promoter        join(287..305,306..311,312..328,329..334,335..360)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    287..305
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    312..330
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             370..381
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             396..413
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttttttggtt tgcagtcaga gtagaataga agtatcaaaa aaagcaccga ctcggtgcca
       61 ctttttcaag ttgataacgg actagcctta ttttaacttg ctatgctgtt ttgaatggtt
      121 ccaacaagat tattttataa cttttataac aaataatcaa ggagaaattc aaagaaattt
      181 atcagccata aaacaatact taatactata gaatgataac aaaataaact actttttaaa
      241 agaattttgt gttataatct atttattatt aagtattggg atcgattccc tatcagtgat
      301 agagattgac atccctatca gtgatagaga tactgagcac atcagcagga cgcactgacc
      361 gaattcatta aagaggagaa aggtgcggcc gcatgcatca ccatcaccat cacatggata
      421 agaaatactc aataggctta gatatcggca caaatagcgt cggatgggcg gtgatcactg
      481 atgaatataa ggttccgtct aaaaagttca aggttctggg aaatacagac cgccacagta
      541 tcaaaaaaaa tcttataggg gctcttttat ttgacagtgg agagacagcg gaagcgactc
      601 gtctcaaacg gacagctcgt agaaggtata cacgtcggaa gaatcgtatt tgttatctac
      661 aggagatttt ttcaaatgag atggcgaaag tagatgatag tttctttcat cgacttgaag
      721 agtctttttt ggtggaagaa gacaagaagc atgaacgtca tcctattttt ggaaatatag
      781 tagat
//
