LOCUS       sequence-108363-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Also referred to as p-Phi-RGNndm-1. Expresses Cas9, tracrRNA and a 
            guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, 
            kanamycin resistance cassette and f1 origin for packaging in M13 
            particles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Citorik RJ, Mimee M, Lu TK
  TITLE     Sequence-specific antimicrobials using efficiently delivered 
            RNA-guided nucleases.
  JOURNAL   Nat Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011.
  PUBMED    25240928
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011."
COMMENT     Sequence Label: pRC319
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(178..196,197..202,203..219,220..225,226..251)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    178..196
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    203..221
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             261..272
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             287..304
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttgaatggt tccaacaaga ttattttata acttttataa caaataatca aggagaaatt
       61 caaagaaatt tatcagccat aaaacaatac ttaatactat agaatgataa caaaataaac
      121 tactttttaa aagaattttg tgttataatc tatttattat taagtattgg gatcgattcc
      181 ctatcagtga tagagattga catccctatc agtgatagag atactgagca catcagcagg
      241 acgcactgac cgaattcatt aaagaggaga aaggtgcggc cgcatgcatc accatcacca
      301 tcacatggat aagaaatact caataggctt agatatcggc acaaatagcg tcggatgggc
      361 ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga
      421 ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc
      481 ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat
      541 ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca
      601 tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt
      661 tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg
      721 aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc
      781 gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag
      841 tgatgtggac aaactattta tccagttggt acaaac
//
