LOCUS       sequence-108364-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Also referred to as p-Phi-RGNndm-1. Expresses Cas9, tracrRNA and a 
            guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, 
            kanamycin resistance cassette and f1 origin for packaging in M13 
            particles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Citorik RJ, Mimee M, Lu TK
  TITLE     Sequence-specific antimicrobials using efficiently delivered 
            RNA-guided nucleases.
  JOURNAL   Nat Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011.
  PUBMED    25240928
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011."
COMMENT     Sequence Label: pRC319
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    64..195
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   196..231
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   262..297
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     primer_bind     complement(717..736)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgccctaga cctagcaccc tgcagaacta tatgattttc cgcagtatat tttagatgaa
       61 gattatttct taataactaa aaatatggta taatactctt aataaatgca gtaatacagg
      121 ggcttttcaa gactgaagtc tagctgagac aaatagtgcg attacgaaat tttttagaca
      181 aaaatagtct acgaggtttt agagctatgc tgttttgaat ggtcccaaaa cgggcagtcg
      241 cttccaacgg tttgatcgtc agttttagag ctatgctgtt ttgaatggtc ccaaaacttc
      301 agcacactga gacttgttga gttccatgtt ttagagctat gctgttttga atggtctcca
      361 ttctctagat gatgattaag gatctatttt tttgggcggg gccgcccaaa aaaatggttc
      421 gcttgctgtc cataaaaccc cgggcagaag atcctgcagg gccttctcca tgcggccgcc
      481 ggtaggctag ggcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      541 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      601 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      661 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      721 ataccaggcg tttccccctg gaagctccct cgtgcgctc
//
