LOCUS       sequence-108365-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector compatible with Flp-mediated (Flp-in) transgene 
            integration that allows for conditional cDNA and Luc expression upon
            integration. The vector contains an FRT site and two mut loxP 
            sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Huijbers IJ, Del Bravo J, Bin Ali R, Pritchard C, Braumuller TM, van
            Miltenburg MH, Henneman L, Michalak EM, Berns A, Jonkers J
  TITLE     Using the GEMM-ESC strategy to study gene function in mouse models.
  JOURNAL   Nat Protoc. 2015 Nov;10(11):1755-85. doi: 10.1038/nprot.2015.114. 
            Epub 2015 Oct 15.
  PUBMED    26492136
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.114"; journalName: "Nat Protoc"; date: 
            "2015-11"; volume: "10"; issue: "11"; pages: "1755-85"
COMMENT     Sequence Label: pFrt-invCAG-ires-Luc
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggccgctagc tacacggcga tcttgccgcc cttcttggcc ttaatgagaa tctcgcggat
       61 cttgcgggcg tccaacttgc cggtcagtcc tttaggcacc tcgtccacga acacaacacc
      121 accgcgcagc ttcttggcgg ttgtaacctg gctggccaca tagtccacga tctccttctc
      181 ggtcatggtt ttaccgtgtt ccagcacgac gactgcggcg ggcagctcgc cggcatcgtc
      241 gtcgggcagg ccggcgaccc cggcgtcgaa gatgttgggg tgttgcagca ggatgctctc
      301 cagttcggct ggggctacct ggtagccctt gtatttgatc aggctcttca gccggtccac
      361 gatgaagaag tgctcgtcct cgtcccagta ggcgatgtcg ccgctgtgca gccagccgtc
      421 cttgtcgatg agagcgtttg tagcctcggg gttgttaacg tagccgctca tgatcatggg
      481 gccacggacg cacagctcgc cgcgctggtt cacacccagt gtcttaccgg tgtccaagtc
      541 caccacctta gcctcgaaga agggcaccac cttgcctact gcgccaggct tgtcgtcccc
      601 ttcgggggtg atcagaatgg cgctggttgt ttctgtcagg ccgtagccct ggcggatgcc
      661 tggtaggtgg aagcgtttgg ccacggcctc acctacctcc ttgctgagcg gcgccccgcc
      721 gctggcgatc tcgtgcaagt tgcttaggtc gtacttgtcg atgagagtgc tcttagcgaa
      781 gaagctaaat agtgtgggca ccagcagggc agattgaatc ttatagtctt gcaagctgcg
      841 caagaatagc tcctcctcga agcggtacat gagcacgacc cgaaagccgc agatcaagta
      901 gcccagcgtg gtgaacatgc cgaagccgtg gtgaaatggc
//
