LOCUS       sequence-108371-        9068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat.
            A retroviral expression vector with the neomycin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, Fujii H
  TITLE     Identification of telomere-associated molecules by engineered 
            DNA-binding molecule-mediated chromatin immunoprecipitation 
            (enChIP).
  JOURNAL   Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038/srep03171.
  PUBMED    24201379
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-8"; volume: "3:3171. doi"; pages: " 
            10.1038/srep03171"
COMMENT     Sequence Label: 3xFN-Tel-TAL/pMXs-neo
FEATURES             Location/Qualifiers
     source          1..9068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             1973..2038
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      2054..2063
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2063..2104
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2117..2137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4957..4977)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4962..5291
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5142..5277
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5648..6442
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5702..5721)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6312..6331
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6513..7106
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7172..7189)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7343..7931)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7423..7442)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8095..8886,8887..8955))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8718..8737
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8956..9060)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 cccagtgtgg tggtacggga attcgtttag tgaaccgtca gaattaacca tggactacaa
     1981 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaagct
     2041 tgcggccggc cgcgccacca tgggaaaacc tattcctaat cctctgctgg gcctggattc
     2101 taccggaggc atggccccta agaaaaagcg gaaggtggac ggcggagtgg acctgagaac
     2161 actgggatat tctcagcagc agcaggagaa gatcaagccc aaggtgagat ccacagtggc
     2221 ccagcaccac gaagccctgg tgggacacgg atttacacac gcccacattg tggccctgtc
     2281 tcagcaccct gccgccctgg gaacagtggc cgtgaaatat caggatatga ttgccgccct
     2341 gcctgaggcc acacacgaag ccattgtggg agtgggaaaa cagtggtctg gagccagagc
     2401 cctggaagcc ctgctgacag tggccggaga actgagagga cctcctctgc agctggatac
     2461 aggacagctg ctgaagattg ccaaaagggg cggagtgacc gcggtggaag ccgtgcacgc
     2521 ctggagaaat gccctgacag gagcccctct gaacctgacc cccgaacagg tggtggccat
     2581 tgccagcaac atcggcggca agcaggccct ggaaaccgtg cagagactgc tgcccgtgct
     2641 gtgccaggcc catggcctga cacctgaaca ggtggtggct atcgcctcta acaacggagg
     2701 aaaacaggct ctggaaacag tgcagcggct gctgcctgtg ctgtgtcagg ctcacggctt
     2761 gactccagaa caggtggtgg ctattgcttc caacaacggg gggaaacagg ccctggaaac
     2821 tgtgcagcgc ctgctgccag tgctgtgcca ggctcacgga ctgacccccg aacaggtggt
     2881 ggccattgcc agcaacaacg gcggcaagca ggccctggaa accgtgcaga gactgctgcc
     2941 cgtgctgtgc caggcccatg gcctgacacc tgaacaggtg gtggctatcg cctctaacgg
     3001 cggaggaaaa caggctctgg aaacagtgca gcggctgctg cctgtgctgt gtcaggctca
     3061 cggcttgact ccagaacagg tggtggctat tgcttccaac ggcgggggga aacaggccct
     3121 ggaaactgtg cagcgcctgc tgccagtgct gtgccaggct cacgggctga cccccgaaca
     3181 ggtggtggcc attgccagca acatcggcgg caagcaggcc ctggaaaccg tgcagagact
     3241 gctgcccgtg ctgtgccagg cccatggcct gacacctgaa caggtggtgg ctatcgcctc
     3301 taacaacgga ggaaaacagg ctctggaaac agtgcagcgg ctgctgcctg tgctgtgtca
     3361 ggctcacggc ttgactccag aacaggtggt ggctattgct tccaacaacg gggggaaaca
     3421 ggccctggaa actgtgcagc gcctgctgcc agtgctgtgc caggctcacg gcctcactcc
     3481 cgaacaggtg gtggccattg ccagcaacaa cggcggcaag caggccctgg aaaccgtgca
     3541 gagactgctg cccgtgctgt gccaggccca tggcctgaca cctgaacagg tggtggctat
     3601 cgcctctaac ggcggaggaa aacaggctct ggaaacagtg cagcggctgc tgcctgtgct
     3661 gtgtcaggct cacggcttga ctccagaaca ggtggtggct attgcttcca acggcggggg
     3721 gaaacaggcc ctggaaactg tgcagcgcct gctgccagtg ctgtgccagg ctcacggact
     3781 gacccccgaa caggtggtgg ccattgccag caacatcggc ggcaagcagg ccctggaaac
     3841 cgtgcagaga ctgctgcccg tgctgtgcca ggcccatggc ctgacacctg aacaggtggt
     3901 ggctatcgcc tctaacaacg gaggaaaaca agcactcgag acagtgcagc ggctgctgcc
     3961 tgtgctgtgt caggctcacg gcttgactcc agaacaggtg gtggctattg cttccaacaa
     4021 cggggggaaa caggccctgg aaactgtgca gcgcctgctg ccagtgctgt gccaggctca
     4081 cgggctgacc cccgaacagg tggtggccat tgccagcaac aacggcggca agcaggccct
     4141 ggaaaccgtg cagagactgc tgcccgtgct gtgccaggcc catggcctga cacctgaaca
     4201 ggtggtggct atcgcctcta acggcggagg aaaacaggct ctggaaacag tgcagcggct
     4261 gctgcctgtg ctgtgtcagg ctcacggctt gactccacag caggtcgtgg caattgctag
     4321 caacggcggc ggacggcccg ccctggagag cattgtggcc cagctgtcta gacctgatcc
     4381 tgccctggcc gccctgacaa atgatcacct ggtggccctg gcctgtctgg gaggcagacc
     4441 tgccctggat gccgtgaaaa aaggactgcc tcacgcccct gccctgatca agagaacaaa
     4501 tagaagaatc cccgagcgga cctctcacag agtggccgat cacgcccagg tggtgagagt
     4561 gctgggattt tttcagtgtc actctcaccc tgcccaggcc tttgatgatg ccatgacaca
     4621 gtttggcatg agcagacacg gactgctgca gctgtttaga agagtgggag tgacagaact
     4681 ggaggccaga tccggaaccc tgcctcctgc ctctcagaga tgggatagga ttctgcaggg
     4741 ttcccgttta attcatcgat agatctgata tcggtaccag tcgactctag aggatcccaa
     4801 ttccagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa
     4861 ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaac
     4921 tcgagcggcc gccagcacag tggtcgatcg acctgtggaa tgtgtgtcag ttagggtgtg
     4981 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5041 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5101 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     5161 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5221 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5281 gcttttgcaa aaagcttcac gctgccgcaa gcactcaggg cgcaagggct gctaaaggaa
     5341 gcggaacacg tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta
     5401 ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg
     5461 gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc
     5521 agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt
     5581 gccgccaagg atctgatggc gcaggggatc aagatctgat caagagacag gatgaggatc
     5641 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     5701 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     5761 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     5821 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     5881 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     5941 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     6001 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     6061 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     6121 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     6181 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     6241 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     6301 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     6361 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     6421 ccttcttgac gagttcttct gagcgggact ctggggttcg catcgtcgac gataaaataa
     6481 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
     6541 aagctagctt aagtaacgcc attttggaag gcatggaaaa atacataact gagaatagag
     6601 aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct
     6661 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg
     6721 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
     6781 ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
     6841 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
     6901 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
     6961 ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
     7021 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
     7081 ctacccgtca gcgggggtct ttcattctgc attaatgaat cggccaacgc gcggggagag
     7141 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7201 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7261 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7321 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7381 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7441 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7501 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7561 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7621 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7681 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7741 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     7801 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7861 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7921 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7981 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8041 tgcggccggc cgcaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8101 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8161 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8221 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8281 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8341 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8401 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8461 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8521 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8581 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8641 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8701 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8761 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8821 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8881 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8941 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9001 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9061 cacatttc
//
