LOCUS       sequence-108379-        8618 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat.
            A retroviral expression vector with the puromycin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8618)
  AUTHORS   Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, Fujii H
  TITLE     Identification of telomere-associated molecules by engineered 
            DNA-binding molecule-mediated chromatin immunoprecipitation 
            (enChIP).
  JOURNAL   Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038/srep03171.
  PUBMED    24201379
REFERENCE   2  (bases 1 to 8618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-8"; volume: "3:3171. doi"; pages: " 
            10.1038/srep03171"
COMMENT     Sequence Label: 3xFN-Tel-TAL/pMXs-puro
FEATURES             Location/Qualifiers
     source          1..8618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             1973..2038
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      2054..2063
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2063..2104
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2117..2137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4953..4973)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4958..5287
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5138..5273
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5297..5896
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5297..5316)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5793..5813
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6123..6592
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6722..6739)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6893..7481)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6973..6992)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7645..8436,8437..8505))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8268..8287
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8506..8610)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 cccagtgtgg tggtacggga attcgtttag tgaaccgtca gaattaacca tggactacaa
     1981 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaagct
     2041 tgcggccggc cgcgccacca tgggaaaacc tattcctaat cctctgctgg gcctggattc
     2101 taccggaggc atggccccta agaaaaagcg gaaggtggac ggcggagtgg acctgagaac
     2161 actgggatat tctcagcagc agcaggagaa gatcaagccc aaggtgagat ccacagtggc
     2221 ccagcaccac gaagccctgg tgggacacgg atttacacac gcccacattg tggccctgtc
     2281 tcagcaccct gccgccctgg gaacagtggc cgtgaaatat caggatatga ttgccgccct
     2341 gcctgaggcc acacacgaag ccattgtggg agtgggaaaa cagtggtctg gagccagagc
     2401 cctggaagcc ctgctgacag tggccggaga actgagagga cctcctctgc agctggatac
     2461 aggacagctg ctgaagattg ccaaaagggg cggagtgacc gcggtggaag ccgtgcacgc
     2521 ctggagaaat gccctgacag gagcccctct gaacctgacc cccgaacagg tggtggccat
     2581 tgccagcaac atcggcggca agcaggccct ggaaaccgtg cagagactgc tgcccgtgct
     2641 gtgccaggcc catggcctga cacctgaaca ggtggtggct atcgcctcta acaacggagg
     2701 aaaacaggct ctggaaacag tgcagcggct gctgcctgtg ctgtgtcagg ctcacggctt
     2761 gactccagaa caggtggtgg ctattgcttc caacaacggg gggaaacagg ccctggaaac
     2821 tgtgcagcgc ctgctgccag tgctgtgcca ggctcacgga ctgacccccg aacaggtggt
     2881 ggccattgcc agcaacaacg gcggcaagca ggccctggaa accgtgcaga gactgctgcc
     2941 cgtgctgtgc caggcccatg gcctgacacc tgaacaggtg gtggctatcg cctctaacgg
     3001 cggaggaaaa caggctctgg aaacagtgca gcggctgctg cctgtgctgt gtcaggctca
     3061 cggcttgact ccagaacagg tggtggctat tgcttccaac ggcgggggga aacaggccct
     3121 ggaaactgtg cagcgcctgc tgccagtgct gtgccaggct cacgggctga cccccgaaca
     3181 ggtggtggcc attgccagca acatcggcgg caagcaggcc ctggaaaccg tgcagagact
     3241 gctgcccgtg ctgtgccagg cccatggcct gacacctgaa caggtggtgg ctatcgcctc
     3301 taacaacgga ggaaaacagg ctctggaaac agtgcagcgg ctgctgcctg tgctgtgtca
     3361 ggctcacggc ttgactccag aacaggtggt ggctattgct tccaacaacg gggggaaaca
     3421 ggccctggaa actgtgcagc gcctgctgcc agtgctgtgc caggctcacg gcctcactcc
     3481 cgaacaggtg gtggccattg ccagcaacaa cggcggcaag caggccctgg aaaccgtgca
     3541 gagactgctg cccgtgctgt gccaggccca tggcctgaca cctgaacagg tggtggctat
     3601 cgcctctaac ggcggaggaa aacaggctct ggaaacagtg cagcggctgc tgcctgtgct
     3661 gtgtcaggct cacggcttga ctccagaaca ggtggtggct attgcttcca acggcggggg
     3721 gaaacaggcc ctggaaactg tgcagcgcct gctgccagtg ctgtgccagg ctcacggact
     3781 gacccccgaa caggtggtgg ccattgccag caacatcggc ggcaagcagg ccctggaaac
     3841 cgtgcagaga ctgctgcccg tgctgtgcca ggcccatggc ctgacacctg aacaggtggt
     3901 ggctatcgcc tctaacaacg gaggaaaaca agcactcgag acagtgcagc ggctgctgcc
     3961 tgtgctgtgt caggctcacg gcttgactcc agaacaggtg gtggctattg cttccaacaa
     4021 cggggggaaa caggccctgg aaactgtgca gcgcctgctg ccagtgctgt gccaggctca
     4081 cgggctgacc cccgaacagg tggtggccat tgccagcaac aacggcggca agcaggccct
     4141 ggaaaccgtg cagagactgc tgcccgtgct gtgccaggcc catggcctga cacctgaaca
     4201 ggtggtggct atcgcctcta acggcggagg aaaacaggct ctggaaacag tgcagcggct
     4261 gctgcctgtg ctgtgtcagg ctcacggctt gactccacag caggtcgtgg caattgctag
     4321 caacggcggc ggacggcccg ccctggagag cattgtggcc cagctgtcta gacctgatcc
     4381 tgccctggcc gccctgacaa atgatcacct ggtggccctg gcctgtctgg gaggcagacc
     4441 tgccctggat gccgtgaaaa aaggactgcc tcacgcccct gccctgatca agagaacaaa
     4501 tagaagaatc cccgagcgga cctctcacag agtggccgat cacgcccagg tggtgagagt
     4561 gctgggattt tttcagtgtc actctcaccc tgcccaggcc tttgatgatg ccatgacaca
     4621 gtttggcatg agcagacacg gactgctgca gctgtttaga agagtgggag tgacagaact
     4681 ggaggccaga tccggaaccc tgcctcctgc ctctcagaga tgggatagga ttctgcaggg
     4741 ttcccgttta attcatcgat agatctgata tcggtaccag tcgactctag aggatcccaa
     4801 ttccagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa
     4861 ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaac
     4921 tcgagcggcc gccagcacag tggtcgacct gtggaatgtg tgtcagttag ggtgtggaaa
     4981 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5041 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5101 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5161 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5221 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     5281 ttgcaaaaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     5341 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     5401 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     5461 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     5521 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     5581 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     5641 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     5701 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     5761 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     5821 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     5881 caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg
     5941 accccatgca tcgtcgacga taaaataaaa gattttattt agtctccaga aaaagggggg
     6001 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
     6061 atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac
     6121 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     6181 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     6241 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
     6301 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
     6361 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
     6421 ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg
     6481 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
     6541 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt cacatgcagc
     6601 atgtatcaaa attaatttgg ttttttttct taagtattta cattaaatgg ccatagttgc
     6661 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     6721 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6781 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6841 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6901 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6961 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7021 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7081 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7141 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7201 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7261 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7321 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7381 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7441 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7501 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7561 tatcaaaaag gatcttcacc tagatccttt tgcggccggc cgcaaatcaa tctaaagtat
     7621 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7681 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7741 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7801 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7861 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7921 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7981 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8041 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8101 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8161 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8221 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8281 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8341 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8401 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8461 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8521 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8581 ttagaaaaat aaacaaatag gggttccgcg cacatttc
//
