LOCUS       sequence-108387-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin 
            S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, 
            Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, 
            Church GM
  TITLE     Highly efficient Cas9-mediated transcriptional programming.
  JOURNAL   Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
  PUBMED    25730490
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312."
COMMENT     Sequence Label: SP-dCas9-VPR
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acttccgttg tgtgatcagt ttggcgttca gcagctgccg ccaataattt ttcattttct
       61 tgacaacttc ttctgagggg acgttatcac tcttccctct agctttatcg gatcttgtca
      121 acactttatt atcaatagaa tcatctttga gaaaagactg gggcacgata gcagccacgt
      181 cgtagtcgga gagccgattg atgtccagtt cctgatccac gtacatgtcc ctgccgttct
      241 gcaggtagta caggtagagc ttctcattct gaagctgggt gttttcaact gggtgttcct
      301 taaggatttg ggaccccagt tcttttatac cctcttcaat cctcttcatc ctttccctac
      361 tgttcttctg tcccttctgg gtagtttggt tctctcgggc catctcgata acgatattct
      421 cgggcttatg ccttcccatt actttgacga gttcatccac gaccttaacg gtctgcagta
      481 ttcccttttt gatagctggg ctacctgcaa gattagcgat gtgctcgtga agactgtccc
      541 cctggccaga aacttgtgct ttctggatgt cctccttaaa ggtgagagag tcatcatgga
      601 tcaactgcat gaagttccgg ttggcaaatc catcggactt aagaaaatcc aggattgtct
      661 ttccactctg cttgtctcgg atcccattga tcagttttct tgacagccgc ccccatcctg
      721 tatatcggcg cctcttgagc tgtttcatga ctttgtcgtc gaagagatga gcgtaagttt
      781 tcaagcgttc ttcaatcatc tccctatctt caaacaacgt aagggtgagg
//
