LOCUS       sequence-108393-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Jan CH, Williams CC, Weissman JS
  TITLE     Principles of ER cotranslational translocation revealed by 
            proximity-specific ribosome profiling.
  JOURNAL   Science. 2014 Nov 7;346(6210):1257521. doi: 10.1126/science.1257521.
            Epub 2014 Nov 6.
  PUBMED    25378630
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2014-11-7"; volume: "346(6210):1257521. doi"; pages: " 
            10.1126/science.1257521. Epub 2014 Nov 6"
COMMENT     Sequence Label: pJW1510
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(115..828)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(115..828)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 aaatcataaa tcataagaaa ttcgcttatt tagaagtggc gcgcctcatt tcataaaaag
       61 gccaaccaaa aacaaaaaaa tagcgatacc aatataaacc atcacgcggc tgtttttgta
      121 caattcatcc ataccatggg taataccagc agcagtaaca aattctaaca agaccatgtg
      181 gtctctcttt tcgtttggat ctttggataa cttagattga taggataagt aatggttgtc
      241 tggtaacaag actggaccat caccaattgg agtattttgt tgataatggt cagctaattg
      301 aacaccacca tcttcaatgt tgtgtctaat tttgaagtta gctttgatac cattcttttg
      361 tttgtcagca gtgatgtaaa cattgtgaga gttatagttg tattccaatt tgtgacctaa
      421 aatgttacca tcttctttaa aatcaatacc ttttaattcg attctattaa ctaaggtatc
      481 accttcaaac ttgacttcag ctctggtctt gtagttaccg tcatctttga aaaaaatagt
      541 tctttcttga acataacctt ctggcatggc agacttgaaa aagtcatgtt gtttcatatg
      601 atctgggtat ctagcaaaac attgcaaacc ataacctaaa gtagtgacta aggttggcca
      661 tggaactggc aatttaccag tagtacaaat caattttaag gtcaatttac cgtaagtagc
      721 atcaccttca ccttcaccgg agacagaaaa tttgtgacca ttaacatcac catctaattc
      781 aaccaaaatt gggacaacac cagtgaataa ttcttcacct ttagacatgt taattaaacc
      841 agcttt
//
