LOCUS       sequence-108395-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAG414 series plasmid with GPD promoter driving expression of 
            dCas9-VPR; cerevisiae vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin 
            S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, 
            Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, 
            Church GM
  TITLE     Highly efficient Cas9-mediated transcriptional programming.
  JOURNAL   Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
  PUBMED    25730490
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312."
COMMENT     Sequence Label: pAG414GPD-dCas9-VPR
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..51
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     35..51
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    59..83
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agttttaaaa caccagaact tagtttcgac ggattctaga actagtggat cccccatcac
       61 aagtttgtac aaaaaagcag gctacaaaat ggacaagaag tactccattg ggctcgctat
      121 cggcacaaac agcgtcggct gggccgtcat tacggacgag tacaaggtgc cgagcaaaaa
      181 attcaaagtt ctgggcaata ccgatcgcca cagcataaag aagaacctca ttggcgccct
      241 cctgttcgac tccggggaga cggccgaagc cacgcggctc aaaagaacag cacggcgcag
      301 atatacccgc agaaagaatc ggatctgcta cctgcaggag atctttagta atgagatggc
      361 taaggtggat gactctttct tccataggct ggaggagtcc tttttggtgg aggaggataa
      421 aaagcacgag cgccacccaa tctttggcaa tatcgtggac gaggtggcgt accatgaaaa
      481 gtacccaacc atatatcatc tgaggaagaa gcttgtagac agtactgata aggctgactt
      541 gcggttgatc tatctcgcgc tggcgcatat gatcaaattt cggggacact tcctcatcga
      601 gggggacctg aacccagaca acagcgatgt cgataaactc tttatccaac tggttcagac
      661 ttacaatcag cttttcgaag agaacccgat caacgcatcc ggagttgacg ccaaagcaat
      721 cctgagcgct aggctgtcca aatcccggcg gctcgaaaac ctcatcgcac agctccctgg
      781 ggagaagaag aacggcctgt ttggtaatct tatcgccctg tcactcgggc tgacccccaa
      841 ctttaaatct aacttcgacc
//
