LOCUS       sequence-108399-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Leu2 marker pXR vector uses for both plasmid assembly in vivo, as 
            well as targeted genomic integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Chou CC, Patel MT, Gartenberg MR
  TITLE     A series of conditional shuttle vectors for targeted genomic 
            integration in budding yeast.
  JOURNAL   FEMS Yeast Res. 2015 May;15(3):fov010. doi: 10.1093/femsyr/fov010. 
            Epub 2015 Mar 2.
  PUBMED    25736914
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2015-05"; volume: "15(3):fov010. doi"; pages: " 
            10.1093/femsyr/fov010. Epub 2015 Mar 2"
COMMENT     Sequence Label: pXRL2
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..53
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    628..661
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agcttcgaca acccttaata taacttcgta taatgtatgc tatacgaagt tattaggtct
       61 agagatcccc gcgcacattt ccccgaaaag tgccacctgg gtccttttca tcacgtacta
      121 taaaaataat tataatttaa attttttaat ataaatatat aaattaaaaa tagaaagtaa
      181 aaaaagaaat taaagaaaaa atagtttttg ttttccgaag atgtaaaaga ctctaggggg
      241 atcgccaaca aatactacct tttatcttgc tcttcctgct ctcaggtatt aatgccgaat
      301 tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc ctgtgatttt acattttact
      361 tatcgttaat cgaatgtata tctatttaat ctgcttttct tgtctaataa atatatatgt
      421 aaagtacgct ttttgttgaa attttttaaa cctttgttta tttttttttc ttcattccgt
      481 aactcttcta ccttctttat ttactttcta aaatccaaat acaaaacata aaaataaata
      541 aacacagagt aaattcccaa attattccat cattaaaaga tacgaggcgc gtgtaagtta
      601 caggcaagcg atgcgagaac ccttaatata acttcgtata atgtatgcta tacgaagtta
      661 ttaggtgata tcagatccac tagctataag gttgatattc tcacgagtaa gcggcacttg
      721 ctacttattg acattgcaga tttttggcta cagaaatagt atattagaga ttataattgc
      781 taatcaaatc aaaatataaa attagtaaac caaaccattt atacccttcc ttagtagtta
      841 tggattgttt tttaatgata tttctg
//
