LOCUS       sequence-108404-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pFA6a Osm1(M33A)-yoEGFP::LEU2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Williams CC, Jan CH, Weissman JS
  TITLE     Targeting and plasticity of mitochondrial proteins revealed by 
            proximity-specific ribosome profiling.
  JOURNAL   Science. 2014 Nov 7;346(6210):748-51. doi: 10.1126/science.1257522.
  PUBMED    25378625
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-11-7- 7"; volume: "346"; issue: 
            "6210"; pages: "748-51"
COMMENT     Sequence Label: pJW1517
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(49..759,760..762))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
ORIGIN
        1 aaatcataaa tcataagaaa ttcgcttatt tagaagtggc gcgccttatt tgtacaattc
       61 atccataccc aacgttatac ccgcagcggt gacaaattct aacaaaacca tgtgatctct
      121 cttttcatta ggatcttttg acaaggcgct ctgtgtagat aagtaatggt tgtctggcaa
      181 tagaacaggt ccatctccga ttggtgtatt ttgttgataa tgatcagcga gttgaacgga
      241 accatcttcg atattatgtc taattttgaa attaactttt atgccgtttt tctgtttatc
      301 tgccatgatg taaacgttat gtgaattgta gttgtattcc aatttgtgac ctaagatatt
      361 accatcttcc ttaaagtcaa taccctttaa ctcaattcta ttaacaagtg tatctccttc
      421 aaacttcact tcagcacgag ttttgtaatt accatcatct ttaaagaaaa tagttctctc
      481 ttgcacatat ccctcaggca tggcagattt gaaaaagtca tgttgtttca tgtggtctgg
      541 gtatcgagaa aaacattgta ctccgtaagt gagagttgta actagagttg gccatgggac
      601 aggcagcttc cctgtggtgc agatgaactt aagtgtaagt ttgccataag ttgcgtcccc
      661 ttcaccctca ccactcacag aaaacttatg cccgtttacg tcgccatcca attctaccag
      721 tattggtacg acacctgtga atagttcctc gccttttgac atgttaatta aaccagcacc
      781 gtcaccgtac aattttgcta tgttatccgc agctgtcttt ccaaagacaa cacactctaa
      841 caaactagat ccacccaatc tgttggctcc atgaacacca cc
//
