LOCUS       sequence-108405-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Trp1 marker pXR vector uses for both plasmid assembly in vivo, as 
            well as targeted genomic integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Chou CC, Patel MT, Gartenberg MR
  TITLE     A series of conditional shuttle vectors for targeted genomic 
            integration in budding yeast.
  JOURNAL   FEMS Yeast Res. 2015 May;15(3):fov010. doi: 10.1093/femsyr/fov010. 
            Epub 2015 Mar 2.
  PUBMED    25736914
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2015-05"; volume: "15(3):fov010. doi"; pages: " 
            10.1093/femsyr/fov010. Epub 2015 Mar 2"
COMMENT     Sequence Label: pXRT1
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(197..871)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
       61 gcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc
      121 atctgtgcgg tatttcacac cgcatagatc ggcaagtgca caaacaatac ttaaataaat
      181 actactcagt aataacctat ttcttagcat ttttgacgaa atttgctatt ttgttagagt
      241 cttttacacc atttgtctcc acacctccgc ttacatcaac accaataacg ccatttaatc
      301 taagcgcatc accaacattt tctggcgtca gtccaccagc taacataaaa tgtaagcttt
      361 cggggctctc ttgccttcca acccagtcag aaatcgagtt ccaatccaaa agttcacctg
      421 tcccacctgc ttctgaatca aacaagggaa taaacgaatg aggtttctgt gaagctgcac
      481 tgagtagtat gttgcagtct tttggaaata cgagtctttt aataactggc aaaccgagga
      541 actcttggta ttcttgccac gactcatctc catgcagttg gacgatatca atgccgtaat
      601 cattgaccag agccaaaaca tcctccttag gttgattacg aaacacgcca accaagtatt
      661 tcggagtgcc tgaactattt ttatatgctt ttacaagact tgaaattttc cttgcaataa
      721 ccgggtcaat tgttctcttt ctattgggca cacatataat acccagcaag tcagcatcgg
      781 aatctagagc acattctgcg gcctctgtgc tctgcaagcc gcaaactttc accaatggac
      841 cagaactacc tgtgaaatta ataacagaca tactc
//
