LOCUS       sequence-108407-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  His3 marker pXR vector uses for both plasmid assembly in vivo, as 
            well as targeted genomic integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Chou CC, Patel MT, Gartenberg MR
  TITLE     A series of conditional shuttle vectors for targeted genomic 
            integration in budding yeast.
  JOURNAL   FEMS Yeast Res. 2015 May;15(3):fov010. doi: 10.1093/femsyr/fov010. 
            Epub 2015 Mar 2.
  PUBMED    25736914
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2015-05"; volume: "15(3):fov010. doi"; pages: " 
            10.1093/femsyr/fov010. Epub 2015 Mar 2"
COMMENT     Sequence Label: pXRH3
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    6..113
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     22..38
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     23..39
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(73..89)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(73..89)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(126..146)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(126..144)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(165..181)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(165..181)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(178..200)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    189..205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(213..219,220..237,238..243))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    258..279
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(396..413)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(647..666)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aattgggtac cgggcccccc ctcgaggtcg acggtatcga taagcttgat atcgaattcc
       61 tgcagcccgg gggatccact agttctagag cggccgccac cgcggtggag ctccagcttt
      121 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
      181 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      241 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      301 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      361 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      421 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      481 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      541 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      601 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      661 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      721 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      781 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      841 cccgaccgct gcgcctta
//
