LOCUS       sequence-108411-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HPH marker pXR vector uses for both plasmid assembly in vivo, as 
            well as targeted genomic integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Chou CC, Patel MT, Gartenberg MR
  TITLE     A series of conditional shuttle vectors for targeted genomic 
            integration in budding yeast.
  JOURNAL   FEMS Yeast Res. 2015 May;15(3):fov010. doi: 10.1093/femsyr/fov010. 
            Epub 2015 Mar 2.
  PUBMED    25736914
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2015-05"; volume: "15(3):fov010. doi"; pages: " 
            10.1093/femsyr/fov010. Epub 2015 Mar 2"
COMMENT     Sequence Label: pXRhMX
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    2..109
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     18..34
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     19..35
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(69..85)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(69..85)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(122..142)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(122..140)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(161..177)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(161..177)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(174..196)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    185..201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(209..215,216..233,234..239))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    254..275
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(392..409)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(643..662)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggtaccggg ccccccctcg aggtcgacgg tatcgataag cttgatatcg aattcctgca
       61 gcccggggga tccactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt
      121 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
      181 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      241 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
      301 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      361 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      421 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      481 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      541 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      601 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      661 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      721 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      781 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacc
//
