LOCUS       sequence-108414-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Kan marker pXR vector uses for both plasmid assembly in vivo, as 
            well as targeted genomic integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Chou CC, Patel MT, Gartenberg MR
  TITLE     A series of conditional shuttle vectors for targeted genomic 
            integration in budding yeast.
  JOURNAL   FEMS Yeast Res. 2015 May;15(3):fov010. doi: 10.1093/femsyr/fov010. 
            Epub 2015 Mar 2.
  PUBMED    25736914
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2015-05"; volume: "15(3):fov010. doi"; pages: " 
            10.1093/femsyr/fov010. Epub 2015 Mar 2"
COMMENT     Sequence Label: pXRkMX
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..59
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    634..667
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agctgaagct tcgacaaccc ttaatataac ttcgtataat gtatgctata cgaagttatt
       61 aggtctagag atccccgcgc acatttcccc gaaaagtgcc acctgggtcc ttttcatcac
      121 gtactataaa aataattata atttaaattt tttaatataa atatataaat taaaaataga
      181 aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt aaaagactct
      241 agggggatcg ccaacaaata ctacctttta tcttgctctt cctgctctca ggtattaatg
      301 ccgaattgtt tcatcttgtc tgtgtagaag accacacacg aaaatcctgt gattttacat
      361 tttacttatc gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc taataaatat
      421 atatgtaaag tacgcttttt gttgaaattt tttaaacctt tgtttatttt tttttcttca
      481 ttccgtaact cttctacctt ctttatttac tttctaaaat ccaaatacaa aacataaaaa
      541 taaataaaca cagagtaaat tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt
      601 aagttacagg caagcgatgc gagaaccctt aatataactt cgtataatgt atgctatacg
      661 aagttattag gtgatatcag atccactagc tataaggttg atattctcac gagtaagcgg
      721 cacttgctac ttattgacat tgcagatttt tggctacaga aatagtatat tagagattat
      781 aattgctaat caaatcaaaa tataaaatta gtaaaccaaa ccatttatac ccttccttag
      841 tagttatgga ttgtttt
//
