LOCUS       sequence-108419-        6086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Met15 marker pXR vector uses for both plasmid assembly in vivo, as 
            well as targeted genomic integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Chou CC, Patel MT, Gartenberg MR
  TITLE     A series of conditional shuttle vectors for targeted genomic 
            integration in budding yeast.
  JOURNAL   FEMS Yeast Res. 2015 May;15(3):fov010. doi: 10.1093/femsyr/fov010. 
            Epub 2015 Mar 2.
  PUBMED    25736914
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2015-05"; volume: "15(3):fov010. doi"; pages: " 
            10.1093/femsyr/fov010. Epub 2015 Mar 2"
COMMENT     Sequence Label: pXRM15
FEATURES             Location/Qualifiers
     source          1..6086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     106..125
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             376..1710
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFKLSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     rep_origin      complement(1815..2270)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1952..1973)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2164..2183
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(2247..2627)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     2396..2418
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2410..2427
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2411..2427
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2437..2456
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2437..2455
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2464..2571
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     2480..2496
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2481..2497
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2531..2547)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2531..2547)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2584..2604)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2584..2602)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2623..2639)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2623..2639)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2636..2658)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2647..2663
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2671..2677,2678..2695,2696..2701))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2716..2737
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2854..2871)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3025..3613)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3105..3124)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3784..4575,4576..4644))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4407..4426
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4645..4736)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    4771..4804
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5379..5412
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcacg
       61 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
      121 ttgtactgag agtgcaccat agccatcctc atgaaaactg tgtaacataa taaccgaagt
      181 gtcgaaaagg tggcaccttg tccaattgaa cacgctcgat gaaaaaaata agatatatat
      241 aaggttaagt aaagcgtctg ttagaaagga agtttttcct ttttcttgct ctcttgtctt
      301 ttcatctact atttccttcg tgtaatacag ggtcgtcaga tacatagata caattctatt
      361 acccccatcc atacaatgcc atctcatttc gatactgttc aactacacgc cggccaagag
      421 aaccctggtg acaatgctca cagatccaga gctgtaccaa tttacgccac cacttcttat
      481 gttttcgaaa actctaagca tggttcgcaa ttgtttggtc tagaagttcc aggttacgtc
      541 tattcccgtt tccaaaaccc aaccagtaat gttttggaag aaagaattgc tgctttagaa
      601 ggtggtgctg ctgctttggc tgtttcctcc ggtcaagccg ctcaaaccct tgccatccaa
      661 ggtttggcac acactggtga caacatcgtt tccacttctt acttatacgg tggtacttat
      721 aaccagttca aaatctcgtt caaaagattt ggtatcgagg ctagatttgt tgaaggtgac
      781 aatccagaag aattcgaaaa ggtctttgat gaaagaacca aggctgttta tttggaaacc
      841 attggtaatc caaagtacaa tgttccggat tttgaaaaaa ttgttgcaat tgctcacaaa
      901 cacggtattc cagttgtcgt tgacaacaca tttggtgccg gtggttactt ctgtcagcca
      961 attaaatacg gtgctgatat tgtaacacat tctgctacca aatggattgg tggtcatggt
     1021 actactatcg gtggtattat tgttgactct ggtaagttcc catggaagga ctacccagaa
     1081 aagttccctc aattctctca acctgccgaa ggatatcacg gtactatcta caatgaagcc
     1141 tacggtaact tggcatacat cgttcatgtt agaactgaac tattaagaga tttgggtcca
     1201 ttgatgaacc catttgcctc tttcttgcta ctacaaggtg ttgaaacatt atctttgaga
     1261 gctgaaagac acggtgaaaa tgcattgaag ttagccaaat ggttagaaca atccccatac
     1321 gtatcttggg tttcataccc tggtttagca tctcattctc atcatgaaaa tgctaagaag
     1381 tatctatcta acggtttcgg tggtgtctta tctttcggtg taaaagactt accaaatgcc
     1441 gacaaggaaa ctgacccatt caaactttct ggtgctcaag ttgttgacaa tttaaagctt
     1501 gcctctaact tggccaatgt tggtgatgcc aagaccttag tcattgctcc atacttcact
     1561 acccacaaac aattaaatga caaagaaaag ttggcatctg gtgttaccaa ggacttaatt
     1621 cgtgtctctg ttggtatcga atttattgat gacattattg cagacttcca gcaatctttt
     1681 gaaactgttt tcgctggcca aaaaccatga gtgtgcgtaa tgagttgtaa aattatgtat
     1741 aaaccgactt tctctcacaa gttatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     1801 aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     1861 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     1921 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     1981 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     2041 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     2101 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     2161 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     2221 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca
     2281 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     2341 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     2401 tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa
     2461 ttgggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatat cgaattcctg
     2521 cagcccgggg gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg
     2581 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     2641 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     2701 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     2761 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     2821 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2881 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2941 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3001 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3061 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3121 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3181 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3241 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3301 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3361 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3421 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     3481 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3541 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3601 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3661 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3721 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3781 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3841 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3901 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3961 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4021 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4081 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4141 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4201 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4261 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4321 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4381 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4441 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4501 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4561 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4621 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4681 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4741 ggttcagctg aagcttcgac aacccttaat ataacttcgt ataatgtatg ctatacgaag
     4801 ttattaggtc tagagatccc cgcgcacatt tccccgaaaa gtgccacctg ggtccttttc
     4861 atcacgtact ataaaaataa ttataattta aattttttaa tataaatata taaattaaaa
     4921 atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa gatgtaaaag
     4981 actctagggg gatcgccaac aaatactacc ttttatcttg ctcttcctgc tctcaggtat
     5041 taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat cctgtgattt
     5101 tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc ttgtctaata
     5161 aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt attttttttt
     5221 cttcattccg taactcttct accttcttta tttactttct aaaatccaaa tacaaaacat
     5281 aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag atacgaggcg
     5341 cgtgtaagtt acaggcaagc gatgcgagaa cccttaatat aacttcgtat aatgtatgct
     5401 atacgaagtt attaggtgat atcagatcca ctagctataa ggttgatatt ctcacgagta
     5461 agcggcactt gctacttatt gacattgcag atttttggct acagaaatag tatattagag
     5521 attataattg ctaatcaaat caaaatataa aattagtaaa ccaaaccatt tatacccttc
     5581 cttagtagtt atggattgtt ttttaatgat atttctgcaa accaaagaaa gattgttatc
     5641 cagatagaat ttagttttga tattcatttt tttgttgaag attgaacgcc atatctgggc
     5701 ctcataattc aaaagacggt gccattatcg gtagcgtttc gcattgtact ggatggcgcg
     5761 ccttcagaaa tttcacagtt gatgaatcga aaagaatggt ctcattgcaa cacgtaaggt
     5821 taagatgtcc ctttttacca ttataggcaa taaatgaatc ataaaacgac cgtatactgg
     5881 tgaaatagta gggagaacga gtacctgtag taaaaagtat aaatcatagt taatcgggca
     5941 atgtccctcg atcaaggagt attgtgtcat gttcgagaca aacgccaaca tttttgtttc
     6001 ttttggacaa atgttgtttg catttatgat ccgttatatt ttgatctaat gtagagttgc
     6061 acgtagttct tactggcaaa gaaatc
//
