LOCUS       sequence-108429-         661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains PLac driving expression of mCherry and PTAN driving 
            expression of GFP. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pZA2[PLac:mCherry,PTAN:GFP]
FEATURES             Location/Qualifiers
     source          1..661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(159..208)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     protein_bind    251..267
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    274..290
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac
       61 tccttgatga tggccatgtt atcctcctcg cccttgctaa ccattatcct ttctccttat
      121 cggtaccttc tatatttaaa caaaattatt tgtagaggct gtttcgtcct cacggactca
      181 tcagaccgga aagcacatcc ggtgacagct ggcgcgccgg tcagtgcgtc ctgctgatgt
      241 gctcagtatc ttgttatccg ctcacaatgt caattgttat ccgctcacaa ttgcggccgc
      301 ggcagttatt ggtgccctta aacgcctggt tgctacgcct gaataagtga taataagcgg
      361 atgaatggca gaaattcgaa agcaaattcg acccggtcgt cggttcaggg cagggtcgtt
      421 aaatagccgc ttatgtctat tgctggttta ccggtttatt gactaccgga agcagtgtga
      481 ccgtgtgctt ctcaaatgcc tgactggtaa caggattagc agagcgaggt atgtaggcgg
      541 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
      601 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      661 c
//
