LOCUS       sequence-108449-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal 3X FLAG fused to wt BirA - mCherry - sec61ß.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Jan CH, Williams CC, Weissman JS
  TITLE     Principles of ER cotranslational translocation revealed by 
            proximity-specific ribosome profiling.
  JOURNAL   Science. 2014 Nov 7;346(6210):1257521. doi: 10.1126/science.1257521.
            Epub 2014 Nov 6.
  PUBMED    25378630
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2014-11-7"; volume: "346(6210):1257521. doi"; pages: " 
            10.1126/science.1257521. Epub 2014 Nov 6"
COMMENT     Sequence Label: pJW1512
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    98..131
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa
       61 attctggccg tttttggctt ttttgttaga cggatccata acttcgtata atgtatgcta
      121 tacgaagtta tgccaccatg tctagactgg acaagagcaa agtcataaac ggagctctgg
      181 aattactcaa tggtgtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg
      241 gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc
      301 tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat
      361 ggcaagactt tctgcggaac aacgccaagt cataccgctg tgctctcctc tcacatcgcg
      421 acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa
      481 atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt
      541 ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat caagtagcaa
      601 aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg
      661 agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat
      721 gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt
      781 ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat atgctgcctg
      841 ctgacgctct tgacgatt
//
