LOCUS       sequence-108463-         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pFTV1 vector contains a thyroxine binding riboswitch, 
            controlling the translation initaition rate of luciferase gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-T4-2
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(131..137,138..153,154..159))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        217..319
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(386..405)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tttctttatg tttttggcgt cttcgaaagc ttccattgtc tccctcctga agcggaggta
       61 cgtcacggtc tcgcctccac agcctcccac aaaccgtgac tcccgcgccg atttcttgag
      121 acgtcccaca cattatacga gccgatgatt aattgtcaac agctcggatc ccctagggaa
      181 ttccgcgctc gaggatatct ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa
      241 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
      301 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
      361 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
      421 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
      481 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg
      541 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
      601 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
      661 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtg
//
