LOCUS       sequence-108464-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 400 / 504, Emission: 515 / 
            517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-Tubulin-C-18
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..753
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(191..209)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 agcagactgg tttagtgacc gtcagatccg ctagcggtcg ccaccatggt gagcaagggc
       61 gaggaggata acatggccat cattaaggag ttcatgcgct tcaaggtgca catggagggg
      121 tccgtgaacg gccacgtgtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      181 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcac ctgggacatc
      241 ctgagccctc agttcatgta cggctccaat gcctacgtga agcaccccgc cgacatcccc
      301 gactacttta agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaaattcgag
      361 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      421 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtgatgca gaagaagacc
      481 atgggctggg aggccctctc cgagcggatg taccccgagg acggcgccct gaagggcgag
      541 gtcaagccca gagtgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
      601 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaaccg caagctggac
      661 atcaccagcc acaacgagga ctacaccatc gtggagcagt acgagagagc cgagggccgc
      721 cactccaccg gcggcatgga cgagctgtac aagtccggac tcagatctgg cagcggtgga
      781 ggcagcgcct ccggcggaag cggaagcgtg cgtgagtgca tctccatcca cgttggccag
      841 gctggtgtcc agattggcaa tgcctgctgg gagctctact gcctggaaca
//
