LOCUS       sequence-108465-         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pFTV1 vector contains a thyroxine binding riboswitch, 
            controlling the translation initaition rate of luciferase gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-T4-2
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      107..193
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(438..457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcagagagat cctcataaag gccaagaagg gcggaaagtc caaattgtaa ccgcacacct
       61 tactggtgtg cggactagtc caactgcaga aaagcggccg cggcatcaaa taaaacgaaa
      121 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
      181 gagtaggaca aatccgccgc cctagagcat gcgcgttcgg ctgcggcgag cggtatcagc
      241 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      301 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      361 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      421 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      481 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      541 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      601 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      661 tcgtcttgag t
//
