LOCUS       sequence-108466-         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pUC19 vector contains a no-aptamer control mRNA encoding 
            luciferase gene. It should be used when testing AEB-TMR7 and 
            AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific 
            ligand effect on luciferase expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: Mishler2014-No-aptamer-controlluciferase-pUC19
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(125..143)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tcatcggctc gtataatgtg tgggactcac tataggtacc ctgagaaggg gcaacaagat
       61 ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatcctctag aggatggaac
      121 cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc
      181 ttttacagat gcacatatcg aggtgaacat cacgtacgcg gaatacttcg aaatgtccgt
      241 tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg
      301 cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc
      361 agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgaacatttc
      421 gcagcctacc gtagtgtttg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa
      481 aaaattacca ataatccaga aaattattat catggattct aaaacggatt accagggatt
      541 tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt
      601 tgtaccagag tcctttgatc gtgacaaaac aattgcactg ataatgaatt cctctggatc
      661 tactgggtta cctaagggtg tggcccttcc gcatagaact gcctgcgtca gattctcgca
      721 tgccagagat ccta
//
