LOCUS       sequence-108467-        2167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 400 / 504, Emission: 515 / 
            517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2167)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-Tubulin-C-18
FEATURES             Location/Qualifiers
     source          1..2167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             20..727
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(165..183)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tccgctagcg gtcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcattaa
       61 ggagttcatg cgcttcaagg tgcacatgga ggggtccgtg aacggccacg tgttcgagat
      121 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      181 caagggcggc cccctgccct tcacctggga catcctgagc cctcagttca tgtacggctc
      241 caatgcctac gtgaagcacc ccgccgacat ccccgactac tttaagctgt ccttccccga
      301 gggcttcaag tgggagcgcg tgatgaaatt cgaggacggc ggcgtggtga ccgtgaccca
      361 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
      421 cccctccgac ggccccgtga tgcagaagaa gaccatgggc tgggaggccc tctccgagcg
      481 gatgtacccc gaggacggcg ccctgaaggg cgaggtcaag cccagagtga agctgaagga
      541 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
      601 gcccggcgcc tacaacgtca accgcaagct ggacatcacc agccacaacg aggactacac
      661 catcgtggag cagtacgaga gagccgaggg ccgccactcc accggcggca tggacgagct
      721 gtacaagtcc ggactcagat ctggcagcgg tggaggcagc gcctccggcg gaagcggaag
      781 cgtgcgtgag tgcatctcca tccacgttgg ccaggctggt gtccagattg gcaatgcctg
      841 ctgggagctc tactgcctgg aacacggcat ccagcccgat ggccagatgc caagtgacaa
      901 gaccattggg ggaggagatg actccttcaa caccttcttc agtgagacgg gcgctggcaa
      961 gcacgtgccc cgggctgtgt ttgtagactt ggaacccaca gtcattgatg aagttcgcac
     1021 tggcacctac cgccagctct tccaccctga gcagctcatc acaggcaagg aagatgctgc
     1081 caataactat gcccgagggc actacaccat tggcaaggag atcattgacc ttgtgttgga
     1141 ccgaattcgc aagctggctg accagtgcac cggtcttcag ggcttcttgg ttttccacag
     1201 ctttggtggg ggaactggtt ctgggttcac ctccctgctc atggaacgtc tctcagttga
     1261 ttatggcaag aagtccaagc tggagttctc catttaccca gcaccccagg tttccacagc
     1321 tgtagttgag ccctacaact ccatcctcac cacccacacc accctggagc actctgattg
     1381 tgccttcatg gtagacaatg aggccatcta tgacatctgt cgtagaaacc tcgatatcga
     1441 gcgcccaacc tacactaacc ttaaccgcct tattagccag attgtgtcct ccatcactgc
     1501 ttccctgaga tttgatggag ccctgaatgt tgacctgaca gaattccaga ccaacctggt
     1561 gccctacccc cgcatccact tccctctggc cacatatgcc cctgtcatct ctgctgagaa
     1621 agcctaccat gaacagcttt ctgtagcaga gatcaccaat gcttgctttg agccagccaa
     1681 ccagatggtg aaatgtgacc ctcgccatgg taaatacatg gcttgctgcc tgttgtaccg
     1741 tggtgacgtg gttcccaaag atgtcaatgc tgccattgcc accatcaaaa ccaagcgcag
     1801 catccagttt gtggattggt gccccactgg cttcaaggtt ggcatcaact accagcctcc
     1861 cactgtggtg cctggtggag acctggccaa ggtacagaga gctgtgtgca tgctgagcaa
     1921 caccacagcc attgctgagg cctgggctcg cctggaccac aagtttgacc tgatgtatgc
     1981 caagcgtgcc tttgttcact ggtacgtggg tgaggggatg gaggaaggcg agttttcaga
     2041 ggcccgtgaa gatatggctg cccttgagaa ggattatgag gaggttggtg tggattctgt
     2101 tgaaggagag ggtgaggaag aaggagagga atactaagga tccaccggat ctagataact
     2161 gatcata
//
