LOCUS       sequence-108469-         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pUC19 vector contains a no-aptamer control mRNA encoding 
            luciferase gene. It should be used when testing AEB-TMR7 and 
            AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific 
            ligand effect on luciferase expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: Mishler2014-No-aptamer-controlluciferase-pUC19
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgtgcggtta caatttggac tttccgccct tcttggcctt tatgaggatc tctctgattt
       61 ttcttgcgtc gagttttccg gtaagacctt tcggtacttc gtccacaaac acaactcctc
      121 cgcgcaactt tttcgcggtt gttacttgac tggcgacgta atccacgatc tctttttccg
      181 tcatcgtctt tccgtgctcc aaaacaacaa cggcggcggg aagttcaccg gcgtcatcgt
      241 cgggaagacc tgccacgccc gcgtcgaaga tgttggggtg ttgtaacaat atcgattcca
      301 attcagcggg ggccacctga tatcctttgt atttaattaa agacttcaag cggtcaacta
      361 tgaagaagtg ttcgtcttcg tcccagtaag ctatgtctcc agaatgtagc catccatcct
      421 tgtcaatcaa ggcgttggtc gcttccggat tgtttacata accggacata atcataggtc
      481 ctctgacaca taattcgcct ctctgattaa cgcccagcgt tttcccggta tccagatcca
      541 caaccttcgc ttcaaaaaat ggaacaactt taccgaccgc gcccggttta tcatccccct
      601 cgggtgtaat cagaatagct gatgtagtct cagtgagccc atatccttgt cgtatccctg
      661 gaagatggaa gcgttttgca accgcttccc cgacttcttt cgaaagaggt gcg
//
