LOCUS       sequence-108471-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 
            gene, using a weak AEB3 promoter. It should be used when testing 
            AEB-DNT3 riboswitch to measure the non-specific DNT effect on mRFP1 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-No-aptamer-control -- AEB3mRFP1
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             156..164
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(179..213)
                     /label=BBa_J23116
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.16"
     promoter        265..367
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(434..453)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tttggtaact ttcagtttag cggtctgggt accttcgtac ggacgacctt caccttcacc
       61 ttcgatttcg aactcgtgac cgttaacgga accttccata cgaactttga aacgcatgaa
      121 ctctttgata acgtcttcag agctcgccat tttttacctc cttatttttt tttctagagc
      181 tagcatagtc cctaggactg agctagctgt caaggatccc ctagggaatt ccgcgctcga
      241 ggatatctgg cgaaaatgag acgttgatcg gcacgtaaga ggttccaact ttcaccataa
      301 tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga
      361 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
      421 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
      481 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
      541 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa
      601 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
      661 aactgaaacg ttttcatcgc tctggagtga ataccacgac ga
//
