LOCUS       sequence-108472-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Derivative of pACYC184 M1uI linkers inserted into EcoRV site 
            inactivating Tc resistance gene. Now Tcs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Donnenberg MS, Calderwood SB, Donohue-Rolfe A, Keusch GT, Kaper JB
  TITLE     Construction and analysis of TnphoA mutants of enteropathogenic 
            Escherichia coli unable to invade HEp-2 cells.
  JOURNAL   Infect Immun. 1990 Jun;58(6):1565-71.
  PUBMED    2160428
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "1990-06"; volume: "58(6)"; pages: "1565-71"
COMMENT     Sequence Label: pCVD433
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(369..374,375..391,392..397))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 cgatgcgtcc ggcgtagagg atccacagga cgggtgtggt cgccatgatc gcgtagtcga
       61 tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg
      121 ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat
      181 agtgactggc gatgctgtcg gaatggacga tgacgcgtca tcccgcaaga ggcccggcag
      241 taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat
      301 gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa
      361 actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattac aacttatatc
      421 gtatggggct gacttcaggt gctacatttg aagagataaa ttgcactgaa atctagaaat
      481 attttatctg attaataaga tgatcttctt gagatcgttt tggtctgcgc gtaatctctt
      541 gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc tgagctacca
      601 actctttgaa ccgaggtaac tggcttggag gagcgcagtc accaaaactt gtcctttcag
      661 tttagcctta accggcgcat gacttcaaga ctaactcctc taaatcaatt accagtggct
      721 gctgccagtg gtgcttttgc atgtctttcc gggttggact caagacgata gttaccggat
      781 aaggcgcagc ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact
      841 gcctacccgg aactgagtgt caggcgtgga atgagacaa
//
