LOCUS       sequence-108474-         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 
            gene, using a weak AEB3 promoter. It should be used when testing 
            AEB-DNT3 riboswitch to measure the non-specific DNT effect on mRFP1 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-No-aptamer-control -- AEB3mRFP1
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      140..211
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      227..254
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      300..386
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(631..650)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg
       61 aacgtgctga aggtcgtcac tccaccggtg cttaataagg ccggccgtgc tagtgtagat
      121 cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      181 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
      241 tgggcctttc tgcgtttata caattgacta gtccaactgc agaaaagcgg ccgcggcatc
      301 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      361 tgaacgctct cctgagtagg acaaatccgc cgccctagag catgcgcgtt cggctgcggc
      421 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      481 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      541 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      601 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      661 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      721 cttc
//
