LOCUS       sequence-108482-         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pFTV1 vector contains a fluoride binding riboswitch, 
            controlling the translation initaition rate of mRFP1 gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 250 to 284)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-F12
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(250..284)
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     promoter        337..439
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(506..525)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tttggtaact ttcagtttag cggtctgggt accttcgtac ggacgacctt caccttcacc
       61 ttcgatttcg aactcgtgac cgttaacgga accttccata cgaactttga aacgcatgaa
      121 ctctttgata acgtcttcag agctcgccat tctgacctcc gacttgggtt ctgtaggcat
      181 catcagctgc tacgggcgca gcggtttgtt aatggaggaa tgccatctcc aattgtattt
      241 ggtctagaag ctagcactgt acctaggact gagctagccg tcaacggatc ccctagggaa
      301 ttccgcgctc gaggatatct ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa
      361 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
      421 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
      481 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
      541 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
      601 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg
      661 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccg
//
