LOCUS       sequence-108487-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Splicing reporter control (no intron) with elements to shorten the 
            half-life of the luciferase protein as well as the luciferase mRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Younis I, Berg M, Kaida D, Dittmar K, Wang C, Dreyfuss G
  TITLE     Rapid-response splicing reporter screens identify differential 
            regulators of constitutive and alternative splicing.
  JOURNAL   Mol Cell Biol. 2010 Apr;30(7):1718-28. doi: 10.1128/MCB.01301-09. 
            Epub 2010 Feb 1.
  PUBMED    20123975
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01301-09"; journalName: "Mol Cell Biol"; date: 
            "2010-04"; volume: "30"; issue: "7"; pages: "1718-28"
COMMENT     Sequence Label: CMV-LUC2CP/ARE
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctctctggct actgagaacc cactgcttac tggcttatcg aaattaatac gactcactat
       61 agggagaccc aagctggcta gttaagcttg gcaatccggt actgttggta aagccaccat
      121 ggaagatgcc aaaaacatta agaagggccc agcgccattc tacccactcg aagacgggac
      181 cgccggcgag cagctgcaca aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc
      241 ctttaccgac gcacatatcg aggtggacat tacctacgcc gagtacttcg agatgagcgt
      301 tcggctggca gaagctatga agcgctatgg gctgaataca aaccatcgga tcgtggtgtg
      361 cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc
      421 tgtggcccca gctaacgaca tctacaacga gcgcgagctg ctgaacagca tgggcatcag
      481 ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa
      541 gaagctaccg atcatacaaa agatcatcat catggatagc aagaccgact accagggctt
      601 ccaaagcatg tacaccttcg tgacttccca tttgccaccc ggcttcaacg agtacgactt
      661 cgtgcccgag agcttcgacc gggacaaaac catcgccctg atcatgaaca gtagtggcag
      721 taccggattg
//
