LOCUS       sequence-108489-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  splicing reporter (with intron) with elements to shorten the 
            half-life of the luciferase protein as well as the luciferase mRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Younis I, Berg M, Kaida D, Dittmar K, Wang C, Dreyfuss G
  TITLE     Rapid-response splicing reporter screens identify differential 
            regulators of constitutive and alternative splicing.
  JOURNAL   Mol Cell Biol. 2010 Apr;30(7):1718-28. doi: 10.1128/MCB.01301-09. 
            Epub 2010 Feb 1.
  PUBMED    20123975
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01301-09"; journalName: "Mol Cell Biol"; date: 
            "2010-04"; volume: "30"; issue: "7"; pages: "1718-28"
COMMENT     Sequence Label: CMV-LUC2CP/intron/ARE
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      120..129
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagtt aagcttggca atccggtact gttggtaaag
      121 ccaccatgga agatgccaaa aacattaaga agggcccagc gccattctac ccactcgaag
      181 acgggaccgc cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca
      241 ccatcgcctt taccgacgca catatcgagg tggacattac ctacgccgag tacttcgaga
      301 tgagcgttcg gctggcagaa gctatgaagc gctatgggct gaatacaaac catcggatcg
      361 tggtgtgcag cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg
      421 gtgtggctgt ggccccagct aacgacatct acaacgagcg cgagctgctg aacagcatgg
      481 gcatcagcca gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg
      541 tgcaaaagaa gctaccgatc atacaaaaga tcatcatcat ggatagcaag accgactacc
      601 agggcttcca aagcatgtac accttcgtga cttcccattt gccacccggc ttcaacgagt
      661 acgacttcgt gcccgagagc ttcgaccggg acaaaaccat cgccctgatc atgaacagta
      721 gtggcagtac cggattgccc aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat
      781 tcagtcatg
//
