LOCUS       sequence-10849-e        1281 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-IHPK2
FEATURES             Location/Qualifiers
     source          1..1281
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             409..420
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 atgagcccag ccttcagggc catggatgtg gagccccgcg ccaaaggcgt ccttctggag
       61 ccctttgtcc accaggtcgg ggggcactca tgcgtgctcc gcttcaatga gacaaccctg
      121 tgcaagcccc tggtcccaag ggaacatcag ttctacgaga ccctccctgc tgagatgcgc
      181 aaattcactc cccagtacaa aggtgtggta tctgtgcgct ttgaagaaga tgaagacagg
      241 aacttgtgtc taatagcata tccattgaaa ggggaccatg gaattgtgga cattgtagat
      301 aattcagact gtgaaccaaa aagtaagctc ctaaggtgga caacaaacaa aaaacatcat
      361 gtcttagaaa cagaaaagac ccctaaggac tgggtgcgtc agcaccgtaa agaggagaaa
      421 atgaagagcc ataagttaga agaagaattt gagtggctaa agaaatctga agtcttgtac
      481 tacactgtag agaagaaggg gaatataagt tcccagctta aacactataa cccttggagc
      541 atgaaatgtc accagcaaca gttacagaga atgaaggaga atgcaaagca tcggaaccag
      601 tacaaattta tcttactgga aaacctgact tcccgctatg aggtgccttg tgtccttgac
      661 ctcaagatgg gcacacgaca acatggtgat gatgcttcag aggagaaggc agccaaccag
      721 atccgaaaat gtcagcagag cacatctgca gtcattggtg tgcgtgtgtg tggcatgcag
      781 gtgtaccaag caggcagtgg gcagctcatg ttcatgaaca agtaccatgg acggaagcta
      841 tcggtgcagg gcttcaagga ggcacttttc cagttcttcc acaatgggcg gtacctgcgc
      901 cgtgaactcc tgggccctgt gctcaagaag ctgactgagc tcaaggcagt gttggagcga
      961 caggagtcct accgcttcta ctcaagctcc ctgctggtca tttatgatgg caaggagcgg
     1021 cccgaagtgg tcctggactc agatgctgag gatttggagg acctgtcaga ggaatcagct
     1081 gatgagtctg ctggtgccta tgcctacaaa cccatcggcg ccagctctgt agatgtgcgc
     1141 atgatcgact ttgcacacac cacctgcagg ctgtatggcg aggacaccgt ggtgcatgag
     1201 ggccaggatg ctggctatat cttcgggctc cagagcctga tagacattgt cacagagata
     1261 agtgaggaga gtggggagtg a
//
