LOCUS       sequence-108492-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 
            gene. It should be used when testing AEB-F12 and AEB-Theo45 
            riboswitches to measure their non-specific ligand effect on mRFP1 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 180 to 214)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-No-aptamer-controlmRFP1
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             156..164
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(180..214)
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     promoter        267..369
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(436..455)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tttggtaact ttcagtttag cggtctgggt accttcgtac ggacgacctt caccttcacc
       61 ttcgatttcg aactcgtgac cgttaacgga accttccata cgaactttga aacgcatgaa
      121 ctctttgata acgtcttcag agctcgccat tttttacctc cttatttttt tttctagaag
      181 ctagcactgt acctaggact gagctagccg tcaacggatc ccctagggaa ttccgcgctc
      241 gaggatatct ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa ctttcaccat
      301 aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag
      361 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
      421 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
      481 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
      541 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg tatggcaatg
      601 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
      661 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
      721 cacatatatt cgcaagatgt ggcgtgttac ggtg
//
