LOCUS       sequence-108494-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for bacterial overexpression of RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pet28a_RBPMS_FL
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             96..128
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             765..782
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(835..853)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 gaatattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatggc tagcatgact ggtggacagc
      121 aaatgggtcg cggatccgaa ttcgagctcc gtcgacccat gaacaacggc ggcaaagccg
      181 agaaggagaa caccccgagc gaggccaacc ttcaggagga ggaggtccgg accctatttg
      241 tcagtggcct tcctctggat atcaaacctc gggagctcta tctgcttttc agaccattta
      301 agggctatga gggttctctt ataaagctca catctaaaca gcctgtaggt tttgtcagtt
      361 ttgacagtcg ctcagaagca gaggctgcaa agaatgcttt gaatggcatc cgcttcgatc
      421 ctgaaattcc gcaaacacta cgactagagt ttgctaaggc aaacacgaag atggccaaga
      481 acaaactcgt agggactcca aaccccagta ctcctctgcc caacactgta cctcagttca
      541 ttgccagaga gccatatgag ctcacagtgc ctgcacttta ccccagtagc cctgaagtgt
      601 gggccccgta ccctctgtac ccagcggagt tagcgcctgc tctacctcct cctgctttca
      661 cctatcccgc ttcactgcat gcccagatgc gctggctccc tccctccgag gctacttctc
      721 agggctggaa gtcccgtcag ttctgctgag cggccgcact cgagcaccac caccaccacc
      781 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      841 agcaataact agcataaccc cttggggcct ctaaacgggt cttga
//
