LOCUS       sequence-108495-         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pFTV1 vector containsa 2,4,dinitrotoluene (DNT) binding 
            riboswitch, controlling the translation initaition rate of mRFP1 
            gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-DNT3
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(287..321)
                     /label=BBa_J23116
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.16"
     promoter        373..475
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(542..561)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tttggtaact ttcagtttag cggtctgggt accttcgtac ggacgacctt caccttcacc
       61 ttcgatttcg aactcgtgac cgttaacgga accttccata cgaactttga aacgcatgaa
      121 ctctttgata acgtcttcag agctcgccat atttgacctc ctctaggtgc tacgaccctt
      181 agccggcgtg ctcccgtaat acggccgggg gcgcacgtac cagagaatag tctaccactt
      241 gtgttatggt accgagctgg aaagtagtgc taatttagcc tctagagcta gcatagtccc
      301 taggactgag ctagctgtca aggatcccct agggaattcc gcgctcgagg atatctggcg
      361 aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc
      421 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga
      481 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
      541 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
      601 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
      661 tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct
      721 ggtgatatgg gatagtgttc acccttgtta caccg
//
